HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Swedlund, H. A. Articles by Chodirker, W. B. Search for Related Content PubMed Articles by Swedlund, H. A. Articles by Chodirker, W. B.

Comparison of Certain Properties of Naturally Occurring Low Molecular Weight M and the M Monomer Derived by Reduction and Alkylation of 19 S M¹

From the Mayo Clinic and Mayo Foundation, Sections of Medicine and Microbiology, Rochester, Minnesota, and the Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York

Abstract

A naturally occurring low molecular weight M protein (M_N) was isolated from the serum of a patient with type I dysgammaglobulinemia by preparative electrophoresis and gel filtration. This protein was compared to the macroglobulin (M₁₉) isolated from the patient's serum and to the M subunit (M_S) prepared by reduction and alkylation of M₁₉. The results indicate that M_N and M_S are similar in size and have the same antigenic determinants. These observations support the concept that the M_N is a naturally occurring subunit of M₁₉ and is very similar to M_S, M₁₉, M_N and M_S were also prepared from the patient's serum after tetanus immunization and were found to bind radiolabeled tetanus toxoid. Most, but not all, controls did not show nonspecific binding of tetanus toxoid. These results suggest but do not conclusively establish the capacity of these M proteins to bind antigen specifically.

Footnotes

¹ A preliminary report of this work was presented at the meeting of the American Academy of Allergy, Palm Springs, California (1), February 18 to 22, 1967. This investigation was supported in part by Research Grants AI-7187, FP-44 and AM-2443 from the National Institutes of Health, Public Health Service.

² Mayo Clinic and Mayo Foundation.

³ University of Rochester School of Medicine and Dentistry.

Present address: Department of Medicine, University of Western Ontario, London, Ontario, Canada.

This article has been cited by other articles:

				R. C. Griggs, W. Strober, and D. E. McFarlin Recurrent Encephalopathy: Associated With Low Molecular Weight {gamma}M in the Serum and Cerebrospinal Fluid Arch Neurol, September 1, 1969; 21(3): 303 - 314. [Abstract] [PDF]