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Studies of the Chicken Immune Response

I. Correlation of the Cellular and Humoral Immune Response¹

From the Department of Biology, Marquette University, Milwaukee, Wisconsin

Abstract

New Hampshire Red chickens (10 to 12 weeks old) were immunized with an intravenous injection of SRBC. By 13 hr, morphologic differentiation was detected in the spleen cell population as indicated by an accumulation of pyroninophilic cells. Differential staining of the nucleic acids indicated that the percentage of plasmacytic cells continued to increase in the spleen for 72 hr, at which time they comprised 17% of the population. The first significant amounts of antibody released by spleen cells were detected by the Jerne plaque technique at this same time suggesting that morphologic differentiation was complete before antibody was released. Maximum antibody-plaque formation occurred at 96 hr and the greatest amount of humoral antibody was detected 72 hr later.

Morphologic changes in the cellular population of the bursa similar to those found in the spleen also occurred after antigenic stimulation. However, no antibody was detected in the bursa. The morphologic changes in the bursa preceded those in the spleen. The possible immunologic implications of these results are discussed.

Footnotes

This study was supported in part by grants from the United States Public Health Service (AM-1024), the American Cancer Society, Milwaukee Division, and in part by Developmental Biology Training Grant 5T1-HD27 from the National Institute of Child Health and Human Development.