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The Journal of Immunology, 1968, 100: 1169-1175.
Copyright © 1968 by The American Association of Immunologists, Inc.
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Preparation of Fluorescent Antibody Reagents Monospecific to Heavy Chains of Human Immunoglobulin1

M. Takahashi, Y. Yagi and D. Pressman

From the Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, New York 142032

Abstract

Fluorochrome-conjugated antibody reagents monospecific to {gamma}-, {alpha}- or µ-heavy chains of human immunoglobulin were prepared from goat antisera by the extensive use of solid immunoadsorbents for the specific purification of desired antibodies and for the removal of other antibodies.

Footnotes

This work was supported in part by Grant No. AI-5158 from the National Institute of Allergy and Infections Diseases.

2 A unit of the New York State Department of Health.






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