HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Claman, H. N. Articles by Brunstetter, F. H. Search for Related Content PubMed Articles by Claman, H. N. Articles by Brunstetter, F. H.

The Response of Cultured Human Thymus Cells to Phytohemagglutinin^1,2,

From the Division of Allergy and Immunology, Department of Medicine, University of Colorado Medical Center, Denver, Colorado 80220

Abstract

1. Studies of human thymus cells in short-term (3 to 91 hr) tissue cultures have shown the stimulation produced by phytohemagglutinin (PHA). This has been demonstrated by increased uptake of isotopically labeled DNA, RNA and protein precursors measured by autoradiography and liquid scintillation counting.

2. The appearance of "blast cells" after culture with PHA has been shown by electron microscopy.

3. There was wide variability from the one thymus cell preparation to another with regard to the dose of PHA and the concentration of cells per culture required for maximal isotope uptake. Thymus cells from older patients appeared to respond better to PHA than did thymus cells from younger patients.

4. A comparison of cultures of peripheral blood leukocytes with cultures of human thymus cells indicates that at least some thymus cell preparations respond to PHA as well as do peripheral blood leukocyte preparations.

Footnotes

Supported in part by: United States Public Health Service AI 00013, AM-10145, American Cancer Society IN 5G #2, and the United States Air Force.

² The views expressed herein are those of the author and do not necessarily reflect the views of the Air Force, the Air University, or the Department of Defense.

This article has been cited by other articles:

				M. A. Levine and H. N. Claman Bone Marrow and Spleen: Dissociation of Immunologic Properties by Cortisone Science, March 13, 1970; 167(3924): 1515 - 1517. [Abstract] [PDF]