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Quantitation of Immunoglobulins and L-Chains by Complement Fixation Tests¹

From the Department of Medicine, University of California at Los Angeles School of Medicine, Los Angeles, California 90024

Abstract

Specific antisera to H chains of G-, A- and M-immunoglobulins and to and light chains were used to quantitate the respective antigens by the sensitive complement fixation technique. Antigens were detectable and were quantitated in hypo--globulinemic fluids, eluates from sensitized red blood cells, saliva, tears and cerebrospinal fluids. Two peaks of complement fixation were obtained with some specimens of tears. It was suggested that this was due to the presence of both serum and secretory A-immunoglobulins in tears. The quantitation of immunoglobulins and L-chains by complement fixation tests appears to be both sensitive and reproducible.

Footnotes

This investigation was supported in part by Research Grant AM-10,000-2 and Training Grant AM 05068 from the National Institutes of Health. Presented in part at the 51st Meeting of the Federation of American Societies for Experimental Biology, Chicago, Illinois, on April 18, 1967.

² Recipient of a Senior Fellowship from the Arthritis Foundation.