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Communications

Tolerance in Vitro: Suppression of Immune Responsiveness to Bovine -Globulin After Injection of Antigen Into Intact Lymphoid Organs

From the Department of Microbiology, Yale University, New Haven, Connecticut 06510

Abstract

Recent studies of immune responses induced in vitro suggest that close contact between phagocytic cells and immunologically competent cells, phagocytosis of antigen, and possibly a "processing step" may be necessary elements of the immunologic induction mechanism (1–3). Several workers have proposed that, in the absence of a phagocytic step, antigen may interact directly with immunologically competent cells and produce tolerance (4, 5). If this is the case, it should be possible to induce tolerance in vitro by exposing lymphoid cells to a non-phagocytizable antigen. We present here data which test this hypothesis and describe some of the conditions under which tolerance can be produced in vitro.

Male Lewis rats were thymectomized as young adults, irradiated (900 r, central axis dose) 3 weeks later, and injected intravenously with 7 to 10 x 10⁸ viable, syngeneic lymph node (LN) and spleen (S) cells, treated in various ways.

Footnotes

Supported in part by United States Public Health Service Training Grant AI 00291 and Research Grants AI 06112 and AI 06455. The data presented here will be included in a thesis submitted to the Graduate School of Yale University in partial fulfillment of the degree of Doctor of Philosophy.