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Separation of New Skin Reactive Antigens from Dwarf Ragweed Pollens¹

From The Research Division of The Sinai Hospital of Detroit, Detroit, Michigan

Abstract

The fractionation of dwarf ragweed pollen extracts has been investigated extensively (1–4) and the results of these studies have been reviewed (5). The principal products, isolated previously, contained one common antigen which was described by King and Norman (3) and which they called antigen E (AgE). King's product, IV-C (3) and Robbins' most recent products, IVRBB and IVRCC (6), contained AgE exclusively as determined by quantitative immunodiffusion methods. On the other hand, Goldfarb's product, pool C, contained 80% AgE. These three sets of products showed significant chemical and physicochemical dissimilarities. The amino acid analyses of IV-C, IVRBB and IVRCC were different in respect to methionine, half-cysteine, proline and tryptophan content. Pool C contained 11% arabinose whereas the other two products were reported to contain 1% or less. On the assumption that arabinose was an impurity, we attempted to extract AgE from pool C by a variety of methods, including the procedure used by King to separate IV-C.

Footnotes

Supported by Grant AI 05386 from The National Institutes of Health.