HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schmidt, N. J. Articles by Dennis, J. Search for Related Content PubMed Articles by Schmidt, N. J. Articles by Dennis, J.

Physical and Immunologic Properties of Rubella Antigens¹

From the Viral and Rickettsial Disease Laboratory, California State Department of Public Health, Berkeley, California 94704

Abstract

The physical and immunologic relationships between infectious rubella virus, the hemagglutinin (HA) and the two complement-fixing (CF) antigens were investigated. The HA eluted from Sephadex G 200 together with the infectious virus and the large-particle CF antigen, and also equilibrated with these two antigens in sucrose gradients at densities of 1.21 to 1.19 g/ml. No HA activity was associated with the small-particle CF antigen. Both HA and infectivity were absorbed into chick erythrocytes, suggesting that the HA is a part of the infectious moiety, but a variable proportion of the large-particle CF antigen was not absorbed onto erythrocytes. Antisera produced by immunization of rabbits with large- or small-particle antigens showed reciprocal crossing in CF tests. Immunization of rabbits with the noninfectious small-particle antigen elicited the formation of neutralizing and HI antibodies as well as CF antibodies. Large- and small-particle CF antigens were also indistinguishable in tests with human sera from natural rubella infections.

Footnotes

The work on which this report is based was supported by Grant AI-01475 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Public Health Service, Department of Health and Welfare.