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Reversible Loss of Activity of the First Component of Complement (C'1) As a Function of Ionic Strength

From the Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

Abstract

Guinea pig C'1a at an ionic strength greater than 0.15 loses up to 90% of its original activity. The rate and extent of inactivation of depend on ionic strength, concentration of C'1a and temperature. The rate and extent of reactivation of C'1a (inactivated by elevated ionic strength) also depend on ionic strength and on the concentration of C'1a but are independent of temperature. The data suggest that the loss of activity of C'1a at high ionic strength is the result of dissociation of C'1a into hemolytically inactive fragments of approximately equal size. C'1a is eluted from EAC'1a by high ionic strength in a hemolytically inactive form. The eluted, inactive C'1a can be reactivated by lowering the ionic strength; the eluted reactivated C'1a cannot be distinguished from untreated C'1a.