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The Journal of Immunology, 1968, 100: 525-529.
Copyright © 1968 by The American Association of Immunologists, Inc.
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Production of Fe Fragments of IgM1

Vincent Yakulis, John Schmale, Nicolas Costea and Paul Heller

From the Veterans Administration West Side Hospital and the Department of Medicine, University of Illinois College of Medicine, Chicago, Illinois

Abstract

Stable Fc fragments were obtained by incubation of human or rabbit IgM with sodium borohydride (NaBH4). Hydrolysis of IgM with papain yielded only small amounts of Fc fragment. Treatment of rabbit IgG with NaBH4 resulted in the formation of Fc fragments which were identical to those obtained by papain digestion. NaBH4 appears to be more suitable than papain for obtaining Fc fragments of IgM.

Footnotes

This investigation was supported by Grant A1-07085 from the National Institutes of Health, United States Public Health Service and by Veterans Administration Medical Research Funds.






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