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Isolation of a Protein Antigen Following Extraction of Lipids from Sheep Red Blood Cell Stroma

I. Hemolytic IgM Primary Response and Hemolytic IgG Secondary Response in Rabbits¹

From the Department of Pathology, Emory University School of Medicine, Atlanta, Georgia

Abstract

Two highly purified protein fractions were isolated after lipid extraction of washed sheep red blood cell stroma. One of these acid soluble proteins has an apparent molecular weight of over 200,000 and elicits a marked circulating hemolytic response in rabbits. Following a single intravenous injection of the high molecular weight antigen in particulate form at pH 7.0, a rise in hemolytic IgM was detected as early as 2 days after sensitization. Hemolytic 7 S -globulins were not detected up to 24 days following primary sensitization with this antigen. Only after challenge of previously sensitized rabbits was circulating hemolytic IgG in rising levels isolated.

Footnotes

This investigation was supported by United States Public Health Service Research Grant HE-08012.

² John and Mary R. Markle Foundation Scholar in Academic Medicine.