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Reaction of Endotoxin and Surfactants

II. Immunologic Properties of Endotoxins Treated with Sodium Deoxycholate

United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Montana

Abstract

Dissociation of Salmonella enteritidis endotoxin into nonpyrogenic subunits of low molecular weight with sodium deoxycholate resulted in reduction of its antigenic and adjuvant properties. Removal of the surfactant by dialysis, permitting reaggregation of the subunits into larger pyrogenic particles, restored antigenic activity. Quantitative precipitation curves confirmed that the molecular species of endotoxin formed after removal of the sodium deoxycholate were much smaller than the original, in the case of phenol-extracted endotoxins, and the same as the original with aqueous ether-extracted endotoxins.