HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ibrahim, A. N. Articles by Hammon, W. McD. Search for Related Content PubMed Articles by Ibrahim, A. N. Articles by Hammon, W. McD.

Application of Immunodiffusion Methods to the Antigenic Analysis of Dengue Viruses¹

II. Immunoelectrophoresis

Department of Epidemiology and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania

Abstract

Six prototypes of dengue viruses, West Nile and Sindbis viruses were cross-reacted in a rather standard type of immunoelectrophoresis test against four rabbit anti-dengue sera representing two closely related pairs as determined by more conventional serology. By this test D-1 and TH-Sman were readily differentiated, each possessing three or four antigens not possessed by the other. One antigen was common to both and to all other dengue protoypes and West Nile virus. A similar differentiation was demonstrated between D-2 and TH-36 and all other viruses employed. One D-2 antiserum demonstrated what may be a subgroup specific dengue antigen shared by all dengues but not by West Nile. The method gives promise of improved differentiation of arboviruses within an antigenic group.

Footnotes

This work was carried out under the sponsorship of the Commission on Viral Infections, Armed Forces Epidemiological Board, and was supported in part by the United States Army Medical Research and Development Command under Contract DA-49-193-MD-2042, and in part by the Public Health Service Training Grant 2 T1 AI110 from the National Institutes of Allergy and Infectious Diseases.

² In part from a thesis submitted by this author in partial fulfillment of the requirements for the Doctor of Science in Hygiene degree.