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Application of Immunodiffusion Methods to the Antigenic Analysis of Dengue Viruses¹

I. Precipitin-in-Gel Double Diffusion in Two Dimensions

Department of Epidemiology and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania

Abstract

The antigenic analysis of dengue viruses was carried out by the micro double immunodiffusion in two dimensions. Results indicated that D-1 Hawaiian strain, and TH-Sman viruses shared at least one common antigen, and each possessed a specific antigen(s). Heterologous cross absorption removed the common, but not the specific antigen(s). It is therefore concluded that these two antigenically related viruses are not identical. Similarly, the antigenic analysis of TH-36 and D-2 New Guinea C strain revealed that these two viruses are not identical.

Footnotes

This work was carried out under the sponsorship of the Commission on Viral Infections, Armed Forces Epidemiological Board, and was supported in part by the United States Army Medical Research and Development Command under Contract DA-49-193-MD-2042, and in part by the Public Health Service Training Grant 2 T1 AI 110 from the National Institutes of Allergy and Infectious Diseases.

² In part from a thesis submitted by this author in partial fulfillment of the requirements for the Doctor of Science in Hygiene.