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Differentiation of Antibody-Forming Cells

I. Ratio of Precursor Cells to Antibody-Forming Cells in the Mouse Spleen^1,2,

Department of Pathology, University of Colorado Medical Center, Denver, Colorado

Abstract

Male LAF₁ mice, 10 hr after irradiation with 750 r, were injected intravenously with normal LAF₁ spleen cells followed 1 day later by an intravenous injection of sheep red blood cells. The recipient mice were sacrificed 6 days after antigen injection and their spleens separated in vitro into two portions: a portion showing precursor cell activity and therefore containing a large number of plaque-forming cells (positive areas), and a portion showing no precursor cell activity and therefore containing very few plaque-forming cells (negative areas). The number of antibody-forming cells and precursor cells in each portion was measured. The ratio of plaque-forming cells to precursor cells was 32:1 in the positive areas and 5.4:1 in the negative areas. These data suggest that all precursor cells are not stimulated maximally by antigen.

Footnotes

¹ A portion of these experiments was presented at a meeting of the Federation of American Societies for Experimental Biology and Medicine, Chicago, Illinois, April 1967.

² This investigation was supported by United States Public Health Service grants AM-08885-03 and CA-5164.