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A Sandwich Technique for the Detection of Myxovirus-Infected Cells Using a Fluorescein-Conjugated Antibody Directed against a Glycoprotein Substrate (Fetuin)¹

Departments of Microbiology and Pathology, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania

Abstract

A two layer "sandwich" technique for the detection of macromolecular synthesis in myxovirus-infected cells has been carried out using a small glycoprotein substrate, fetuin, followed by an outer layer of antifetuin antibody labeled with fluorescein isothiocyanate. This method appears to offer a high degree of sensitivity and permits one to use a single fluorescent label for the identification of myxovirus-infected cells.

Footnotes

Supported by Grant AI-06015 from the National Institute of Allergy and Infectious Diseases.

² Medical Student Trainee (University of Chicago) supported by Grant 2 TI-AI-203-06 from the National Institutes of Health.