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Rhinoviruses: Basis for a Numbering System

1. HeLa Cells for Propagation and Serologic Procedures¹

Children's Hospital Research Foundation and the Departments of Pediatrics and Medical Microbiology, Ohio State University College of Medicine, Columbus, Ohio

Abstract

HeLa cell cultures were used to propagate 128 rhinovirus strains. Rhinovirus preparations derived from HeLa cells possessed higher infectivity titers than those obtained from human diploid cells. Using identical conditions, attempts to propagate rhinoviruses in HeLa and H.Ep2 cells demonstrated the superiority of the HeLa cell system.

Standardization of antisera and identification of viruses were complicated by the presence of cytotoxic factor(s) in many of the antisera. Cytotoxicity could be reduced by treatment with either HeLa cell sediment or human liver powder. The evidence suggests that cytotoxic factor(s) probably are antibodies directed against host cell antigens.

Footnotes

This work was supported by Public Health Service Research Contract PH 43-65-562 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.