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A Study of Agglutinable Antigens of Bacillus Influenzae

I. Methods of Preparing and Preserving Antigens of B. Influenzae, and their Effects Upon Specific Agglutination

From the Laboratory of Bacteriology of the Philadelphia General Hospital, Philadelphia, Pennsylvania

Abstract

Attempts to classify hemophilic bacilli of the Bacillus influenzae group have yielded very contradictory results in the hands of different investigators. Biologic reactions have failed to do more than define the general characters of the family (1). Attempted immunologic classification by agglutination and by the absorption of agglutinins has not been successful (2). The methods employed in conducting these immunologic tests have been nearly as diverse as the results obtained with them.

Agglutination tests have been conducted with saline suspensions of bacteria grown on solid medium and with broth culture suspensions. Incubation for agglutination has been conducted at 37°, at 45° and at 55°C. The time intervals at these temperatures have been from two to twenty-four hours, and often followed by a period in the refrigerator before the final reading of the results. Account has not been taken of the hydrogen ion concentration at which the reactions were studied.